Bovine lactoferrin ELISA kit

Bovine lactoferrin ELISA kit is a competitive ELISA kit to detect bovine lactoferrin in milk, milk powder and lactoferrin raw materials

Bovine lactoferrin is produced on an industrial scale from cheese whey or skim milk. Ingestion of bovine lactoferrin (LF) has been reported to show anti-infective, anti-cancer, and anti-inflammatory effects. In particular, it has become evident that oral bLF had a beneficial effect on infections of both digestive and nondigestive tract tissue in various animal models. Furthermore, the effects of bLF have been indicated in clinical studies on patients with Helicobacter pylori infection, chronic hepatitis C, tinea pedis, and other diseases. Immunomodulation in the intestine and systemic sites has been suggested to mediate the protective effects of oral bLF against infection.

As an important nutrient, bovine lactoferrin is added into baby formula quite frequently. It is also a quality indicator of bovine colostrum. 

This bovine lactoferrin ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the chloramphenicol will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of bovine lactoferrin contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of bovine lactoferrin in the sample.

Key facts of the Bovine lactoferrin ELISA kit

• Ready to use ELISA kit based on competitive Immunoassay
• Total time required only 75min
• Highly specific to bovine lactoferrin
• Applicable for both milk and milk powder

Bovine Lactoferrin ELISA Kit parameters

• Sensitivity: 0.1 mg/L (ppm)
• Stability: 12 months when stored properly
• Accuracy: 80%-120%

Bovine Lactoferrin ELISA Kit Components

• Precoated microwell, 96wells
• bLF standard series, 0.1 ppm - 8.1 ppm
• Enzyme conjugate
• TMB Substrate
• Stop solution (not available when shipping by air)
• Sample buffer
• Wash buffer

For more information about the kit, please feel free to contact us.

Further readings

• Tomita, M., et al. "Bovine lactoferrin and lactoferricin derived from milk: production and applications." Biochemistry and Cell Biology 80.1 (2002): 109-112.
• Tsuda, Hiroyuki, et al. "Milk and dairy products in cancer prevention: focus on bovine lactoferrin." Mutation Research/Reviews in Mutation Research 462.2-3 (2000): 227-233.